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Journal of Biological Science, Technology and Management Vol. 5 Issue 1 2023

Vol. 5 No. 1 (2023)

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Articles Published in This Issue

Explore peer-reviewed research articles published in this issue.

researchpp. 128–132

Evaluation of Stevia rebaudiana Leaf-Axillary Shoot Formation, Cultured in MS Medium Supplemented with IAA-BAP and MS Medium Supplemented with Kinetin

Stevia rebaudiana leaves can be used as a sweetener alternatives because they contain steviol glycoside derivative compounds, including steviosides and rebaudioside-A. Propagation of Stevia is more optimally carried out using in vitro culture when compared to conventional propagation through seeds or cuttings. This study aimed to evaluate the formation and growth of Stevia shoots and leaves in MS medium containing a mixture of IAA and BAP with MS medium containing kinetin only, as well as evaluating the use of a liquid medium containing kinetin. Stevia was initiated from apical shoot then…

Keywords
axillary shoot BAP IAA kinetin Stevia rebaudiana
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researchpp. 133–141

Behavioral Response of Komodo Dragons (Va- ranus komodoensis OUWENS, 1912) During Mating and Nesting Periods towards Tourist Presence in Loh Buaya, Komodo National Park

It has been recognized in many studies that wildlife tourism practices might generate a negative impact on wildlife, particularly during the reproductive period. Some wildlife may lower their sensitivity towards tourist presence, for instance in Komodo. Understanding to what extenthabituation occurs in Komodo would be necessary for tourism management in Komodo National Park (KNP). Therefore, this study aimed to identify the response of Komodo to tourist presence during mating and nesting activities. The observation was conducted in Loh Buaya, which is one of the tourism sites in KNP. Komodo’s…

Keywords
wildlife tourism Komodo reproductive behavior habituation
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researchpp. 142–148

Primer Design and Optimization of Annealing Temperature for Analysis of Glutathione Reductase Gene Expression in Rice (Oryza sativa L.)

Glutathione Reductase (GR) belongs to the NADPH-dependent flavoprotein oxidoreductase family and is found in both prokaryotes and eukaryotes. The GR gene is considered to play a key role in the elimination of oxidative reaction products by looking at the level of gene expression of GR rice in dealing with drought stress using qPCR. One of the important steps to develop a specific, effective and efficient qPCR is the primer design. Several studies analyzing GR gene expression in rice have also designed primers. However, the primer still lacks an ideal characteristic of primer, as it still has…

Keywords
annealing temperature glutathione reductase primer design rice
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researchpp. 149–161

Stand Structure and Composition and Model for Estimating Stand Volume Potential at the Citragaluh Sustainable Community Forest Management Unit, Subang Regency, West Java

In general, community forest management is still limited to the management of individual farmers so that it affects diversity, especially in the form of stands. This study aimed to explore the stand structure and composition characteristics and develop a model for estimating the potential stand volume at the Citragaluh community forest management unit with 55 observation plots based on a combination of slope and density classes. The data taken includes slope; plant species; plant coordinates; planting pattern and spacing; tree diameter, tree height, and canopy density. The results of plot…

Keywords
community forest agroforestry stand structure stand composition stand volume potential
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researchpp. 162–167

Short Note on Asteraceae as Traditional Food and Medicinal Plants in Cihanjawar Village, Purwakarta Regency, West Java

Asteraceae is known as the largest family of flowering plants. Despite some species members being invasive plants, these species are often adopted and utilized by local community groups for food, traditional medicine, and other uses. In our ethnobotanical study of Asteraceae, we identified ways a local Sundanese community group in West Java utilizes a diverse range of species in the family for different purposes. Our study focuses on a Sundanese village called Cihanjawar, located in the regency of Purwakarta, using ethnobotany and ethnomedicine approaches. People of Cihanjawar utilize some…

Keywords
Asteraceae ethnobotany ethnomedicine lalapan Sundanese
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