Embryo Incision as a New Technique for Double Seedling Production of Indonesian Elite Coconut Type âKopyorâ
One of the present major limitations of seedling production of kopyor type coconut using embryo culture is that only one seedling can be produced from a single embryo. Therefore, we report on the development of a new breakthrough technique for the production of double seedlings from a single embryo. The technique consists of four steps, viz. (i) germination; (ii) incision; (iii) splitting; and (iv) recovery. A histological study was carried out on the development of the halved embryo into a new shoot. The best recovery process was obtained when the incised embryo was split into two and…





