Vol. 38 No. 2 (2013)

Soursop (Annona muricata L.) leaves were used as a traditional drug for many diseases such as cancer. Statistical data from one cancer hospital in Jakarta in the period of 2005 and 2007 showed that prostate cancer has become one of ten common cancer cases in ambulatory patients. This research was conducted to investigate the potency of an herbal drug for prostate cancer. The soursop leaves were extracted by maceration and fractionated by liquid-liquid extraction. The activity of all samples was then tested by Brine Shrimp Lethality Test (BSLT) and MTT assay using prostate cell line, LNCaP.…

Temu kunci (Beosenbergia pandurata Roxb.) is traditionally used to treat various diseases, and antioxidants are one of their utilization. The aim of this study was to isolate and identify the antioxidant compounds of temu kunci rhizomes. Temu kunci rhizomes was extracted by maceration with ethanol 95%. Etanol extract was then fractionated by liquid-liquid extraction, vacuum liquid chromatography, and classical column chromatography. Monitoring and testing the antioxidant activity used thin-layer chromatography (TLC) with 0.2% DPPH (1,1-diphenyl-2-picrylhydrazyl) reagent. Purity test was…

The research to compare the antimicrobial activity profile of Piper betle L. leaf, Pluchea indica L. leaf, and Citrus aurantifolia [Christm.] Swingle fruit against bacteria isolated from axillary sweat of six probandus having axillary odor problem had been done. Eight pure bacterial isolates came from this process, and then identified with Gram stain and several biochemical reactions. Staphylooccus aureus, S. epidermidis, and S. haemolyticus, known as bacteria that usually present in a large amount at human axilla skin with odor problem, were used also in this research. The tested plants were…

The flavonoid compound was successfully isolated from jackfruit leaves (Artocarpus heterophyllus Lamk.). Extraction was done by maceration with methanol-water (9:1), followed by methanol-water (1:1) as a solvent. Hydrolyzed extract was obtained by adding of 2NHCl, then extracted with ethyl acetate and monitored by thin layer chromatography (TLC) with nhexane-ethyl acetate (3:5) as a mobile phase. Separation was performed by column chromatography, followed by preparativethin layer chromatography. Purity of isolates was determined by 2-dimensional thin layer chromatography. Characterization of…

In this study, antioxidant activity and antocyanin derivatives study of extract of red katuk (Euphorbia cotinifolia L.) leaves was done. Antioxidant activity was done by DPPH (2,2-diphenyl 1-picrylhydrazyl) method using ultraviolet-visible spectrophotometer and thin layer chromatography. The result showed that methanol extract had antioxidant activity with IC50 value 33.13 μg/mL. Vitamin C as comparative substance had IC50 value was 5.24 μg/mL. From the acidified methanol extract, two anthocyanin derivatives have been isolated by preparative paper chromatography and characterized by…

The antidiarrheal activities of water extracts of guava leaves (Psidium guajava L.) and water extracts of green tea leaves (Camellia sinensis L.) combination against castor oil-induced diarrhea and intestinal transit time method have been determined in Swiss Webster male mice. Twenty five male Swiss Webster mice weighing around 20-35 g were divided randomly into 5 groups for each method. The first group as a control was given gom 2% po, the second group was treated with loperamide hydrochloride 0.52 mg/kg body weight po. The third, fourth and fifth groups were treated with water extract of…