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Journal of Biological Science, Technology and Management Vol. 3 Issue 1 2021

Vol. 3 No. 1 (2021)

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Articles Published in This Issue

Explore peer-reviewed research articles published in this issue.

researchpp. 1–8

Analysis of MeEf1A6 Gene Promoter Activity with In-vitro and In-vivo using Transient and Stable Expression Techniques in Tobacco Plant (Nicotiana tabacum)

The promoter is a part of the gene that functions in carrying out the gene expression, and its work activity becomes a matter of concern to ensure that expression works effectively. MeEF1A6 (Manihot esculenta Elongation Factor 1 Alfa - 6) is a promoter derived from cassava plants (Manihot esculenta). In previous studies, the MeEF1A6 promoter was successfully isolated, introduced, and characterized into the pBI121 plasmid, replacing the CaMV35S promoter. This study aims to analyze the activity of MeEF1A6 promoters in-vivo and in-vitro by using transient and transgenic techniques in tobacco…

Keywords
Promoter Transformation MeEF1A6
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researchpp. 18–27

Analysis of Bacterial Community level Physiological Profiling on the Fermentation of Traditional Pliek u using BIOLOG EcoPlates

Pliek u is an Acehnese traditional condiment made from fermented coconut (Cocos nucifera) endosperm. The traditional pliek u fermentation process typically involves a diverse bacterial community. This research aimed to discover the physiological profile of the bacterial community diversity in pliek u fermentation. BIOLOGTM EcoPlates was used to obtain the physiological functions of the bacterial community during the pliek u fermentation process. The bacteria were then isolated from EcoPlate substrate to determine the predominant microorganism. Results from the analysis showed that the value…

Keywords
Pliek u BIOLOG EcoPlate Fermentation Microbial Community
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researchpp. 28–41

CO2 Emission and Absorption Estimation in Bandung City by Implementing CO2 Emission Rate Reduction Simulation Using the Stella Program

Bandung CO2 emissions continue to increase in line with its population. The emissions source comes from the industrial, transportation, Liquefied Petroleum Gas (LPG), household, and livestock sectors, whereas CO2 absorption only comes from vegetation through photosynthesis. High CO2 emissions could decrease air quality and reduce environmental health. This study aims to estimate the amount of CO2 emissions and their absorption in Bandung by implementing CO2 Emission Rate Reduction Simulation (CERRS). The simulation comprises four scenarios, namely substitution of vehicle fuel and the…

Keywords
CO2 Emissions CO2 Absorptions CERRS
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researchpp. 42–55

Optimasi Frekuensi Pemberian Pakan terhadap Performa Pertumbuhan Udang Putih (Litopenaeus vannamei) pada Fase Grow-out

Udang putih (Litopenaeus vannamei) merupakan komoditas industri perikanan yang produksinya berkembang pesat sejak awal 1970-an. Indonesia sebagai salah satu sumbangsih pasar ekspor udang putih terbesar di dunia terus berusaha untuk menginkatkan produksi. Dalam meningkatkan produksi udang putih para pelaku budidaya terus diterjang permasalahan seperti performa pertumbuhan yang tidak optimal. Salah satu faktor terbesar yang berpengaruh terhadap performa pertumbuhan serta efisiensi produksi udang putih adalah manajemen pakan yang optimal. Manajemen pakan meliputi frekuensi pemberian pakan,…

Keywords
FCR feed growth Litopenaeus vannamei survival frequency
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researchpp. 9–17

Selection of Indonesian Medicinal Plant Active Compounds as Inhibitor Candidates of Oncoproteins E6 and E7 Human Papillomavirus Type 16 by Molecular Docking

Cervical cancer cases caused by infection with Human Papillomavirus (HPV), especially HPV 16 (60.5% of cases) continue to increase every year with a high mortality rate. The current anti-cancer drugs were not only specifically targeting cancer cells, but healthy cells and can cause serious side effects. Therefore, it is necessary to find safer alternative therapies, e.g., using active compounds from natural products. The purpose of this study was to find the active compounds of Indonesian medicinal plants potentially as an inhibitor of oncoprotein E6 and E7 HPV 16, the main protein causing…

Keywords
cervical cancer E6 HPV16 E7 HPV16 molecular docking inhibitor
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