Genetic Diversity of Indigofera tinctoria L. in Java and Madura Islands as Natural Batik Dye based on Inter-simple Sequence Repeat Markers
Indigo leaves (Indigofera tinctoria L.) are utilized as a source of batik dye in Java and Madura islands, Indonesia. Batik makers from the islands collect indigo leaves from wild plants. Continuous collection of wild plants from their natural habitat may decrease their diversity. The present research was conducted to analyze the genetic diversity and population structure of I. tinctoria L. from Java and Madura. 55 indigo accessions were collected from 10 locations in Java and Madura. The indigo DNA was extracted and amplified using 15 ISSR primers. A total of 123 bands showed 50-90%…





