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Journal of Biological Science, Technology and Management Vol. 7 Issue 1 2025

Vol. 7 No. 1 (2025)

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Articles Published in This Issue

Explore peer-reviewed research articles published in this issue.

researchpp. 289–299

Effectiveness and Public Perception of Synthetic and Natural-Based Mosquito Repellents Against Aedes aegypti in Indonesia

Since 1968, dengue fever cases in Indonesia have persisted despite various vector control efforts. One of the most common and accessible methods of personal protection is the use of mosquito repellents. This study evaluated public perceptions of mosquitoes and repellents, their willingness to pay (WTP) for these products, and the efficacy of synthetic and natural-based repellents against Aedes aegypti, considering variations in mosquito strain and age. A Knowledge, Attitude, and Practices (KAP) analysis was conducted to assess public understanding and behaviors, utilizing the Analytical…

Keywords
Aedes aegypti Strain Vector Control Strategy Repellent Natural-based repellent DEET
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researchpp. 300–307

Melon Waste Ecoenzyme: An Eco-friendly Liquid Organic Fertilizer for Vegetative Growth of Melon (Cucumis melo L. var. Merlin)

The utilization of melon plant waste, such as fruit peels, leaves, and stems, can be done by processing it into eco-enzyme, which is then used as liquid organic fertilizer. This study aims to assess the effect of eco-enzyme on the vegetative growth of melon plants. The randomized block design (RBD) method was used with seven treatments and four replications, including P0 (negative control), P1 (10 mL/L), P2 (20 mL/L), P3 (30 mL/L), P4 (40 mL/L), P5 (50 mL/L), and P6 (positive control). Observations were made every 7 days for 30 days, with parameters including plant height, leaf area, number…

Keywords
Eco-enzyme Melon Organic Waste Vegetative Growth
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researchpp. 308–317

Analysis of Quercetin from Allium cepa L. Skin Waste in Inhibiting α-Amylase: An Alternate Therapeutic Drug to Treat Diabetes

Type 2 diabetes is a chronic metabolic disease characterized by an increase in blood sugar due to insulin resistance as well as the decline of insulin production by the pancreas. Quercetin is a flavonoid secondary metabolite that could be utilized as an α-amylase inhibitor and can potentially be used as a therapeutic drug in type 2 diabetes treatment by inhibiting starch hydrolysis in the human body. This research aims to determine the quercetin yield of Allium cepa L. skin waste, evaluate the α-amylase inhibitory properties of the extracted quercetin, and delve into the molecular mechanism…

Keywords
α-amylase inhibition quercetin type 2 diabetes
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researchpp. 318–325

Characterization of Pathogenic Bacteria Causing Bacterial Wilt Disease of Kepok Banana in Manokwari, West Papua

Kepok bananas, including their processed derivatives, serve as a vital alternative staple food alongside rice and contribute significantly to food security, particularly in the Manokwari region. Maintaining the health of banana plants is essential for preserving global food diversity. Ensuring their sustainable availability is crucial; however, this is increasingly threatened by the emergence of banana diseases. The rapid spread of banana diseases was first reported in 2018 in the SP IV Bowi Subur area of Masni District, and by 2019, similar cases were documented in the Anday area of South…

Keywords
Banana diseases Ralstonia syzygii subsp. celebensis Anday
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researchpp. 326–333

Optimization of PCR Primers for Detection of Extended-Spectrum Beta-Lactamase Targeting CTX-M and TEM Genes

Extended-Spectrum Beta Lactamase (ESBL) is an enzyme that inhibit the activity of third-generation cephalosporin antibiotics. CTX-M and TEM are the genes encoding the ESBLs. The horizontal spread of these gene from one bacteria to another leads to increased bacterial resistance. Molecular-based bacterial identification methods such as Polymer Chain Reaction are methods that are currently used because they provide faster and more specific results. Therefore, in this study, a method for identifying bacteria that produce ESBL was created by targeting CTX-M and TEM genes. In this study, two pairs…

Keywords
Polymer Chain Reaction primer optimization in silico analysis CTX-M TEM genes
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